In vitro regeneration of Haloxylon ammodendron

Haloxylon ammodendron (C.A.Mey) Bunge is one of the important species arid desert vegetation in China, and it also an ideal material for studying plant stress resistance, which plays extremely role protecting ecosystems preventing land desertification. However, there are relatively few studies on regeneration fusiform, genetic transformation system has not been successfully reported, restricts in-depth study molecular mechanism shuttle propagation resistance. In present study, seeds, hypocotyls, cotyledons, cotyledon nodes, terminal buds fixed were used as explants, a set tissue culture was established by inducing adventitious buds, bud rooting transplanting. The results showed that amongst different H. i.e., cotyledons last induced budding effect better. optimal medium clandine segment differentiation 0.5 mg·L-1 NAA+0.5 6-BA rate high, reaching 61.90%, 1/2 MS+1 NAA +1 IBA + 1 IAA with 50%. this will provide theoretical basis ammodendron.